Development of mature citrus tissue transformation technology
Principal Investigator Affiliation:
United States Department of Agriculture - Agricultural Research Service
Citrus transformation technology is difficult, time consuming and limited to a few non-elite cultivars. Without improvements to the citrus transformation process when a cure for HLB is discovered a majority of the existing citrus cultivars will lose commercial viability. Previously, though a CRB funded proposal (5200-165), we studied novel ways of increasing citrus transformation efficiency through techniques such as wounding, surfactants, hormone type/timing, media additives, genes that induce growth, genes that inhibit cell death and unique wild Agrobacterium. We propose combining these techniques for additive enhancing effects towards improving mature citrus transformation.