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Development of mature budwood transformation technology

Project ID:

5200-165

Principal Investigator:

James Thomson

Principal Investigator Affiliation:

United States Department of Agriculture - Agricultural Research Service

Co-Investigators:

N/A

Collaborators:

  • Ed Stover
  • Eliezer S. Louzada
  • Mikeal Roose
  • Gitta Coaker

Start Date:

2016

Estimated Duration:

4 year(s)

Completed Date:

10/2020

Annual Funding:

N/A

The lack of HLB resistance in major citrus scion types combined with the limited time frame for developing resistance has compelled industry interest in identifying transgenic solutions. The objective of this research is to develop requisite molecular tools for the efficient transformation of mature citrus cultivars with native citrus resistance genes when possible. Recombinase technology will be included allowing future gene stacking and marker gene removal for improved public perception. All research will now be conducted at the USDA in CA to avoid further delays in interstate transfer of transgenic citrus lines produced.

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