Development of "all Plant" transgenic citrus with potential broad spectrum disease resistance using gene gun

Transgenic plants containing any sequence (promoter, terminator, others) of a plant pest or that was genetic modified using Agrobacterium- mediated genetic transformation are considered by the Animal and Plant Health Inspection Service (APHIS) as regulated articles. In this project we will produce genetic modified plants containing only DNA from plants, devoid of any markers (nptll gene, GUS, others), and the transformation method will not involve any plant pathogen, we will be using biolistic transformation method (gene gun). The end results of this project will be citrus scion and rootstocks genetic modified to have broad spectrum disease resistance and that will be immediately available for testing without the need of regulation from APHIS.