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Past Research – Page 4

2005 Edwin Civerolo (Database for Microarray-based Detection Systems)

Development and Management of a Genomics Database for
Microarray-based Detection Systems for Citrus Pathogens

The specific objective is to develop and manage a database of genomics information related to citrus pathogens and infection and to citrus pathogen-host interactions. The main targeted citrus pathogens for this project are Xylella fastidiosa (Xf), citrus tristeza virus (CTV) and huanglongbing (HLB).

2005 J.E. Adaskaveg (Hyphoderma Gummosis)

Etiology, Epidemiology, and Management of Hyphoderma Gummosis

Hyphoderma gummosis, caused by the Basidiomycota fungus Hyphoderma sambuci, was first observed in Tulare County in 2000. In surveys done in collaboration with farm advisors Ben Faber and Neil O’Connell, the disease was subsequently found in numerous lemon orchards in Ventura, Tulare, as well as Riverside and San Luis Obispo Counties.

2005 J.E. Adaskaveg (Septoria Spot of Citrus)

Biology and Management of Septoria Spot of Citrus

Septoria spot of citrus caused by Septoria citri is found in many citrus-producing countries around the world. It is a sporadic disease in California with most reports coming from Tulare Co. and Fresno Co. The pathogen is present on leaves and twigs in many orchards, and fruit infections probably occur commonly. Disease from these quiescent infections, however, only develops when plant tissues become senescent or environmental conditions predispose the host to infection.

2005 Marylou Polek (Tristeza Virus Strains)

Biological Characterization of Naturally Occurring Citrus Tristeza Virus Strains in California Citrus and Maintenance of the Isolate Collection

This project is not a typical research project. Rather, it is one that largely supports the research of several laboratories including those located at the University of California at Davis, UC Riverside, USDA Parlier, USDA Riverside, Thomas Jefferson University, and AgDia (a private company). This report will summarize the services and materials provided to these laboratories.

2005 Peggy A. Mauk ( Mandarin Trial for the California Desert)

Mandarin Trial for the California Desert

Mandarin production has been a stable source of income for growers in the Coachella Valley for over three decades. Growers continue to have success with Fairchild Mandarin as well as with Minneola Tangelo. However, in order to stay competitive with domestic and global fresh citrus markets, there is a need for seedless mandarin varieties suitable for the California desert climate.

2005 Jose X. Chaparro ( Breeding a Red-Fleshed Mandarin )

Breeding a Red-Fleshed Mandarin (Candidate Gene Analysis)

The title of this project is “Breeding a Red Fleshed Mandarin,” subtitled “Candidate Gene Analysis.” The project has been a collaboration between Jose ChaparroJose Chaparro at the University of Florida, Andrew Breksa at the USDA-ARS facility in Albany, California, and Greg McCollum at the USDA-ARS-HRL at Ft. Pierce, Florida.

2005 Bryce W. Falk (Controlling CTV )

Controlling Citrus Tristeza Virus by Rootstock Delivery of a CTV RNA Silencing Signal

We completed the fourth and final year of our CRB-funded effort to develop Citrus tristeza virus (CTV) RNA silencing as an approach to confer systemic resistance against CTV.

2005 Mikeal L. Roose and Timothy J. Close ( Microarrays for Gene Expression)

Microarrays for Gene Expression and Mapping in Citrus

This project is best considered as a supplement to projects 5200-121 (EST Libraries and Bioinformatics) and 5200-125 (Genetic Maps of Sweet Orange and Trifoliate Orange) which use a new tool, microarrays, to study patterns of gene expression and map genes. Those projects are funded by CRB with matching funds from UC Discovery, but we are not permitted to spend those Discovery funds to purchase the microarrays necessary for the research. This CRB grant supplemented other UC funds and allowed us to purchase the chips needed to conduct the other projects.

2005 Mikeal L. Roose and Timothy J. Close (Genetic Maps)

Genetic Maps of Sweet Orange and Trifoliate Orange

This project is a collaboration involving the Roose and Close labs at UCR and the Fred Gmitter and Jose Chaparro labs at the University of Florida. The main goal is to develop genetic linkage maps of sweet orange and trifoliate orange. Genetic linkage maps are used to improve the efficiency of breeding and to identify genes that, if altered using biotechnology, would confer desired traits on the plant. The maps developed under this project will be the most detailed and accurate available for citrus. CRB funding enables us to obtain matching funds from the UC Discovery program (about $1.35 for each $1 of CRB funds).

2005 Timothy J. Close and Mikeal L. Roose ( EST Libraries)

EST Libraries and Bioinformatics for California Citrus

This project provides foundational information in the area of “Genomics”. The information is about the genes, proteins and metabolic pathways of citrus. This information facilitates other research and practical applications. Research applications include identification of genes that underlay pathogen resistance and development of transgenic strategies to investigate gene-trait relationships and develop new varieties. Practical applications include genetic markers for breeding and variety identification, and new methods to measure fruit quality.