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Past Research – Page 10

2008 Joseph Morse (Management of Thrips )

Management of Citrus Thrips and Bean Thrips

At the advice of Citrus Research Board members, we merged our research on citrus thrips with work requested by industry to improve the systems approach used to reduce bean thrips levels on citrus shipped from California to Australia.

2008 Edwin E Lewis (Nematodes Agains Diaprepes)

Assessing the Efficacy of Entomopathogenic Nematodes Against Diaprepes abbreviatus in California Soils and Climates

Three specific objectives have been addressed in the first two years of the project: 1). We have collected and analyzed the following physical and chemical properties of soil from sites of citrus production throughout California: % of sand, silt and clay; pH, electrical conductivity, organic matter and moisture retention, % water, and water potential; 2). We have assayed all of these samples for the presence of native populations of entomopathogenic nematodes; and 3). We have tested the efficacy of entomopathogenic nematodes in each of the tested soils.

2008 Bruce McPheron (Tephritid Barcoding Initiative)

Tephritid Barcoding Initiative

The goal of this project was to develop a new diagnostic tool for pest fruit flies based upon DNA sequences. DNA diagnostics has emerged as a powerful tool for identifying unknown samples, particularly life stages for which there are not reliable morphological characteristics to use (in this case, eggs and larvae, for example).

2008 Beth Grafton Cardwell(Pest Management Infrastructure )

Pest Management Infrastructure

This Citrus Research Board funding supports three Staff ResearchAssociates located at theKearneyAgriculturalCenter and Lindcove Research and Extension Center. During 2007- 08, these SRAs conducted projects to improve integrated pest management of various pests including citrus peelminer, citrus leafminer, California red scale, citrus red mite, and citricola scale.

2008 CCQC Jim Cranney

California Citrus Quality Council (CCQC) Quality Assurance Program

As specifically provided for in the California Citrus Improvement Program marketing order, this ongoing Quality Assurance Program is conducted by the California Citrus Quality Council (CCQC) under an operating agreement with the California Citrus Research Board.

2008 CCPP Georgios Vidalakis

Citrus Clonal Protection Program (CCPP)

The California Citrus Clonal Protection Program (CCPP) is a part of the University of California, Riverside, Department of Plant Pathology and Microbiology. The CCPP is a cooperative program with the California Department of Food and Agriculture (CDFA), the United States Department of Agriculture Animal and Plant Health Inspection Service (USDA-APHIS) and the citrus industry of the state of California represented by the California Citrus Nursery Board (CCNB) and the Citrus Research Board (CRB), which is the primary supporting agency.

2008 Robert Cary (Novel Immunocapture …..)

Novel Immunocapture Technology for Field Deployable Nucleic Acid-Based Detection of Citrus Pathogens

The primary hypothesis guiding this project is that a highly simplified and field deployable sample preparation system can be realized using low cost and easily used lateral flow chromatography technologies. Specifically, we proposed the development of a nucleic acid analysis system making use of Lateral Flow Microarray technology (LFM) and supporting field deployable sample preparation and amplification methods. Toward this end we are developing and testing lateral flow chromatographic immuno-capture methods, novel passive fluid exchange systems, and integration of these systems to form the basis of a field deployable diagnostic platform.

2008 N W Schaad (Cultivation and Sequencing of HLB)

Cultivation and Rapid Detection of the Causal Agent of Huanglongbing Disease of Citrus

Huanglongbing (HLB), an insect-transmitted disease of citrus, also known as citrus greening, results in a green blotchy mottle on foliage, small, lopsided fruit with tree decline and eventual tree death. The causal organism has been identified as a bacterium, however no organism had been previously cultured. The protocol for identifying disease agents is to follow Koch’s postulates: isolate the pathogen from the diseased host and grow the pathogen in culture, use the culture to infect a healthy host plant and observe typical symptoms of the disease, and re-isolate the pathogen from symptomatic tissue and grow in culture.

2008 Kentaro Inoue (Molecular Analysis of Greening….)

Molecular Analysis of Degreening and Regreening in ValenciaOrange

Mature orange fruits change their peel color from green to orange during ripening. This degreening is an important process to determine the commercial quality of fruits. After reaching the full-ripe stage, some late season varieties such as Valencia turn their fruit peel color from orange to green. The regreened fruits are ripe inside, but cannot sell well due to their unripe-like appearance. The funded project aims to understand the molecular bases of this peel color development of citrus fruit, and to use the outcomes to develop efficient strategies to enhance color development and prevent regreening from happening.

2008 Jianchi Chen (Fingerprinting of HLB Strains)

Genetic Diversity and Fingerprinting of HLB Bacteria

This is the first year report of the two-year project on the genetic diversity and fingerprinting of Huanglongbing- associated bacteria. The project involves three objectives: 1). Analyses of historically and currently available literature data on HLB and identify important issues to address; 2). Study what microorganisms are involved in HLB in addition to what we currently know; and, 3). Use genomic information to study the variation of HLB-associated bacteria and their source of origins.